Volume 12: 2022 - Article VII

Abstract
This study investigated the effects of hepatoprotection by Pterocarpus santalinoides methanol leaf extract during carbon tetrachloride-induced sub-acute hepatotoxicity on thiopentone sleeping time in albino rats. Leaves of Pterocarpus santalinoides were dried under shade, pulverized into powdered form, extracted by cold maceration with 80% methanol, and used for the study. Thirty adult male albino rats randomly assigned to six groups (A – F) of five rats each were used for the study. Carbon tetrachloride was administered to groups A – E rats at the dose of 1 ml/kg intraperitoneally at three-day intervals from day 0 to day 12 of the experiment (total of five times). Group A rats were treated with 10 ml/kg distilled water (negative control), groups B, C and D rats were treated with 50, 250 and 500 mg/kg extract, respectively, Group E rats were treated with 100 mg/kg silymarin as positive control, while Group F rats were given only 10 ml/kg distilled water (normal control). Treatment with the extract, silymarin and distilled water placebo were done per os, twice daily, from day 1 (a day after the first carbon tetrachloride injection) up to day 15 (end of the study). Blood samples were collected from all the rats on day 15 for liver function tests. After the blood sample collection, a single intra-peritoneal injection of 50 mg/kg sodium thiopentone was given to all the rats to induce narcosis. The duration of sleep (sleeping time) for each rat in all the groups was recorded. Results showed that the group D rats given 500 mg/kg extract had significantly (p < 0.05) shorter sleep duration when compared to the negative control (Group A) and the group treated with 50 mg/kg extract (Group B). Also, co-treatment at the dose of 250 and 500 mg/kg extract and 100 mg/kg silymarin (Group E) significantly (p < 0.05) protected hepatocellular integrity and ameliorated impaired hepatic excretory function induced by carbon tetrachloride. It was concluded that the extract at 500 mg/kg dose significantly shortened sodium thiopentone sleeping time in albino rats with hepatopathy caused by carbon tetrachloride.
Keywords: Pterocarpus santalinoides leaf extract, CCl4-induced hepatotoxicity, Sodium thiopentone-induced sleeping time, Rats.
How to cite this article:
Ihedioha TE, Asuzu IU, Anaga AO and Ihedioha JI (2022) Hepatoprotection by Pterocarpus santalinoides methanol leaf extract during carbon tetrachloride induced subacute hepatotoxicity: effects on thiopentone-induced sleep duration in albino rats. Journal of Veterinary and Applied Sciences, 12: 46 – 53.

HEPATOPROTECTION BY Pterocarpus santalinoides METHANOL LEAF EXTRACT DURING CARBON TETRACHLORIDE INDUCED SUBACUTE HEPATOTOXICITY: EFFECTS ON THIOPENTONE-INDUCED SLEEP DURATION IN ALBINO RATS

Thelma E. Ihedioha¹, Isaac U. Asuzu¹, Aruh O. Anaga¹, John I. Ihedioha²,
¹Department of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria.
²Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria.

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